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Advanced Biomaterials and Devices in Medicine
December 2014, Volume 1, Issue 1, pp 18-27
Multilayered bioactive composite coatings with drug delivery capability by electrophoretic deposition combined with layer-by-layer deposition
Q. Chen1, Y. Liu2, Q.Q. Yao3, S.S. Yu3, K. Zheng1, M. Pischetsrieder2, A.R. Boccaccini1*
1 Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, 91058 Germany
2 Department of Chemistry and Pharmacy, Food Chemistry, University of Erlangen-Nuremberg, Erlangen, 91052 Germany
3 Institute of Advanced Materials for Nano-Bio Applications, School of Ophthalmology & Optometry, Wenzhou Medical University, Zhejiang, 325027 China
* Corresponding author Prof. Dr. Aldo R. Boccaccini, aldo.boccaccini@ww.uni-erlangen.de
Abstract
A combination of electrophoretic deposition and layer-by-layer deposition was applied to fabricate multilayered bioactive coatings consisting of hydroxyapatite and biodegradable polymers including alginate, chitosan and polyvinyl alcohol. The bottom layer, namely hydroxyapatite/alginate composite, was deposited onto stainless steel substrates by one-step electrophoretic deposition. The second layer made of alginate and polyvinyl alcohol was also deposited by electrophoretic deposition, and then layer-by-layer was introduced for the deposition of chitosan and alginate layers. A range of characterization methods including scanning electron microscopy, Fourier transform infrared spectroscopy and laser profilometer were used to evaluate the compositional and microstructural properties of the deposited multilayer coatings. The coatings showed a stable degradation rate, and superior bioactivity due to the presence of hydroxyapatite in the bottom layer. Furthermore, the burst release of lysozyme, which was selected as a model actibacterial agent in this study, was significantly suppressed and a sustained release up to 14 days was obtained. Cell viability and antibacterial tests of lysozyme-loaded multilayer coatings were carried out to confirm the suitability of the proposed coating system for multifunctional modification of opthopaedic implants and to validate the coating techniques involved.
Keywords: electrophoretic deposition, multilayer, hydroxyapatite, lysozyme, polyelectrolyte, drug delivery
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